The Frauen DFB-Pokal 1999–2000 was the 20th season of the cup competition, Germany's second-most important title in women's football. The first round of the competition was held from 22–25 August 1999. In the final which was held in Berlin on 6 May 2000 FFC Frankfurt defeated Sportfreunde Siegen 2–1, thus defending their title from the previous season thus claiming their second title.
1st round
* Windhorst Drosendorf and Ahrbach withdrew their teams. Nürnberg and Saarbücken thus advanced to the next round.
2nd round
* Hertha Zehlendorf withdrew their team. Brauweiler Pulheim thus advanced to the next round.
Quarter-finals
Semi-finals
Final
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| | League competitions | | Men | | Level 1 & 2 |  | 
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 | Level 3 |  | 
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 | Level 4 | 
Baden-WürttembergBayernHessenHamburg/Schleswig-HolsteinNiedersachsen/BremenNordrheinNordostSüdwestWestfalen
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 | Women |  | 
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 | Cup competitions |  | 
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 | European competitions |  | 
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 | Related to national teams |  | 
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| | Team seasons |  | Men's Bundesliga |  | 
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 | Men's 2. Bundesliga | 
Alemannia AachenTennis Borussia BerlinVfL BochumChemnitzer FC1. FC KölnFortuna KölnEnergie CottbusSpVgg Greuther FürthHannover 96Karlsruher SCMainz 05Waldhof MannheimBorussia Mönchengladbach1. FC NürnbergRot-Weiß OberhausenKickers OffenbachStuttgarter KickersFC St. Pauli
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