RLS
RLS may refer to:
Education
- Royal Latin School, a grammar school in Buckingham, England
 - Stevenson School, formerly Robert Louis Stevenson School, California
 
Science and technology
- Recursive least squares filter, in minimisation
 - Regularized least squares, for solving least-squares problems
 - Reef Life Survey, a marine monitoring program in Tasmania
 - Restless legs syndrome, a medical disorder
 
Other uses
- Real-life slash, a subgenre of real person fiction
 - Rial (disambiguation), plural abbreviation Rls, Middle Eastern currency units
 - Robert Louis Stevenson, Scottish writer