The 2012 Duquesne Dukes football team represented Duquesne University as a member of the Northeast Conference during the 2012 NCAA Division I FCS football season. Led by eighth -year head coach Jerry Schmitt, the Dukes compiled and overall record of 5–6 with a mark of 3–5 in conference play, tying for sixth place in the NEC. Duquesne play their home games at Arthur J. Rooney Athletic Field in Pittsburgh.
Schedule
| Date | Time | Opponent | Site | TV | Result | Attendance |
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| September 1 | 6:00 pm | at No. 8 Old Dominion* | | | L 23–57 | 19,818 |
| September 8 | 12:00 pm | Dayton* | | | W 17–7 | 1,902 |
| September 15 | 1:00 pm | at Valparaiso* | | | W 45–17 | 2,177 |
| September 22 | 1:00 pm | at Bryant | | | W 35–21 | 2,958 |
| September 29 | 1:00 pm | Saint Francis (PA) | - Arthur J. Rooney Athletic Field
- Pittsburgh, PA
| | W 24–21 | 2,641 |
| October 13 | 12:00 pm | at Central Connecticut | | | L 31–38 | 4,013 |
| October 20 | 12:00 pm | Sacred Heart | - Arthur J. Rooney Athletic Field
- Pittsburgh, PA
| | W 35–3 | 2,215 |
| October 27 | 12:00 pm | Monmouth | - Arthur J. Rooney Athletic Field
- Pittsburgh, PA
| | L 27–28 | 1,897 |
| November 3 | 3:30 pm | at Robert Morris | | FCS, ROOT, ESPN3 | L 13–17 | 2,028 |
| November 10 | 12:00 pm | Albany | - Arthur J. Rooney Athletic Field
- Pittsburgh, PA
| | L 31–38 | 2,193 |
| November 17 | 12:00 pm | at Wagner | | | L 17–23 | 2,692 |
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References
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National championship seasons in bold |